Dianthramide glucosides from tissue cell cultures of Delphinium staphisagria L.

Tissue cell cultures of Delphinium staphisagria L. produced three dianthramide glucosides N-(2′-β-glucopyranosylsalicyl)-5- hydroxyanthranilic acid methyl ester, N-(2′-β-glucopyranosyl- 5′-methoxysalicyl)-5-hydroxyanthranilic acid methyl ester and N-(2′-β-glucopyranosyl-5′-hydroxysalicyl)-5-hydroxy-6- methoxyanthranilic acid methyl ester, together with known methyl esters of N-salicylanthranilic acid and N-(2′-β-glucopyranosyl-5′- hydroxysalicyl)-5-hydroxyanthranilic acid. Structures of the glucosides were established by MS, 1-D and 2-D NMR techniques.