Genetic diversity of the malaria vaccine candidate plasmodium falciparum merozoite surface protein-3 in a hypoendemic transmission environment

Stephen J. Jordan, Oralee H. Branch, Jean Carlos Castro, Robert A. Oster, Julian C. Rayner

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The N-terminal domain of Plasmodium falciparum merozoite surface protein-3 (PfMSP3) has been excluded from malaria vaccine development largely because of genetic diversity concerns. However, no study to date has followed N-terminal diversity over time. This study describes P1MSP3 variation in a hypoendemic longitudinal cohort in the Peruvian Amazon over the 2003-2006 transmission seasons. Polymerase chain reaction was used to amplify the N-terminal domain in 630 distinct P. falciparum infections, which were allele-typed by size and also screened for sequence variation using a new high-throughput technique, denaturing high performance liquid chromatography. PfMSP3 allele frequencies fluctuated significantly over the 4-year period, but sequence variation was very limited, with only 10 mutations being identified of 630 infections screened. The sequence of the P1MSP3 N-terminal domain is relatively stable over time in this setting, and further studies of its status as a vaccine candidate are therefore warranted.

Original languageEnglish
Pages (from-to)479-486
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume80
Issue number3
DOIs
StatePublished - Mar 2009

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