Improving the performance of transglutaminase-crosslinked microparticles for enteric delivery

Fernando Tello, Ana S. Prata, Rodney A.F. Rodrigues, Adilson Sartoratto, Carlos R.F. Grosso

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Various agents for cross-linking have been investigated for stabilizing and controlling the barrier properties of microparticles for enteric applications. Transglutaminase, in addition to being commercially available for human consumption, presents inferior cross-linking action compared to glutaraldehyde. In this study, the intensity of this enzymatic cross-linking was investigated in microparticles obtained by complex coacervation between gelatin and gum Arabic. The effectiveness of cross-linking in these microparticles was evaluated based on swelling, release of a model substance (parika oleoresin: colored and hydrophobic) and gastrointestinal assays. The cross-linked microparticles remained intact under gastric conditions, whereas the uncross-linked microparticles have been dissolved. However, all of the microparticles have been dissolved under intestinal conditions. The amount of oily core that was released decreased as the amount of transglutaminase increased. For the most efficient microparticles (50 U/g of protein), the performance was improved by increasing the pH of cross-linking from 4.0 to 6.0, resulting in a release of 17.1% rather than 32.3% of the core material. These results were considerably closer to the 10.3% of core material released by glutaraldehyde-cross-linked microparticles (1 mM/g of protein).

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalFood Research International
Volume88
DOIs
StatePublished - 1 Oct 2016
Externally publishedYes

Keywords

  • Complex coacervation
  • Cross-linking
  • Enzyme
  • Gastrointestinal resistance
  • Microencapsulation

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