The efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for visceral leishmaniasis diagnosis is due to low levels of cross-reactivity

Viviana Pinedo-Cancino, Norival Kesper, Clara Lućia Barbiéri, José Angelo Lauletta Lindoso, Eufrosina Setsu Umezawa

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The analysis of promastigote excreted-secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the Leishmania species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the L. (L.) chagasi ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. The analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the L. (L.) chagasi ESA-ELISA and 9.4% reacted with the ESA isolated from L. (L.) amazonensis and L. (V.) braziliensis, whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). The ESA-blot of L. (L.) chagasi tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.

Original languageEnglish
Pages (from-to)559-565
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume88
Issue number3
DOIs
StatePublished - Mar 2013
Externally publishedYes

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