TY - JOUR
T1 - Genetic diversity and population structure of endangered rosewood from the peruvian Amazon using issr markers
AU - Guizado, Stalin Juan Vasquez
AU - Nadeem, Muhammad Azhar
AU - Ali, Fawad
AU - Barut, Muzaffer
AU - Habyarimana, Ephrem
AU - Gómez, Tedi Pacheco
AU - Santillan, Jhon Antoni Vargas
AU - Canales, Esperanza Torres
AU - Gómez, Juan Carlos Castro
AU - Chung, Gyuhwa
AU - Baloch, Faheem Shehzad
N1 - Publisher Copyright:
© 2020, Instituto Nacional de Pesquisas da Amazonia. All rights reserved.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed the presence of maximum variability within populations (88%). The Structure algorithm, neighbor joining and principal coordinate analysis (PCoA) grouped the 90 rosewood accessions into three main populations (A, B and C). Diversity indices at the inter-population level revealed a greater genetic diversity in population A, due to higher gene flow. The neighbor-joining analysis grouped populations A and B, while population C was found to be divergent at the inter population level. We concluded that population A reflects higher genetic diversity and should be prioritized for future management and conservation plans.
AB - Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed the presence of maximum variability within populations (88%). The Structure algorithm, neighbor joining and principal coordinate analysis (PCoA) grouped the 90 rosewood accessions into three main populations (A, B and C). Diversity indices at the inter-population level revealed a greater genetic diversity in population A, due to higher gene flow. The neighbor-joining analysis grouped populations A and B, while population C was found to be divergent at the inter population level. We concluded that population A reflects higher genetic diversity and should be prioritized for future management and conservation plans.
KW - Aniba rosaeodora
KW - Endangered species
KW - Gene flow
KW - Germplasm
KW - Molecular characterization
UR - http://www.scopus.com/inward/record.url?scp=85093876653&partnerID=8YFLogxK
U2 - 10.1590/1809-4392201902203
DO - 10.1590/1809-4392201902203
M3 - Article
AN - SCOPUS:85093876653
SN - 0044-5967
VL - 50
SP - 204
EP - 212
JO - Acta Amazonica
JF - Acta Amazonica
IS - 3
ER -